2014-2015 Price List (Exclusive of Shipping)

Baculovirus synthesis from sequence:

$1495+$1.50 per amino acid

Synthesize a baculovirus-optimized shuttle vector for your desired protein, generate high purity baculovirus, amplify virus to 250 ml high titer stock and determine the titer by limiting dilution.

Subclone cDNA from your plasmid into a baculovirus transfer vector, cotransfect with viral DNA and amplify virus.

$2295 (additional charge for linkers if necessary or for special transfer vectors).

Transfect and amplify viral transfer vector you make:

$995

The transfer vector you provide will be used to develop a high purity virus and the virus amplified to 250 ml high titer virus, and the titer determined.


Clone your virus by limiting dilution.

$595

Your virus will be cloned by limiting dilution on 96 well dishes. 5 clones will be used to test infect one T-25 flask each and the pellet (and media if appropriate) will be shipped to you for analysis. The chosen clone will be amplified to 250ml and the titer will be determined.

Electrophoresis and Western blot of cells infected with viral clones.

$495 plus cost of first antibody
$250 for additional gels run at the same time

Your clonal pellets will be applied to an SDS-PAGE gel, blotted and probed with an antibody you provide or using any commercially available antibody. Detection by ECL. Maximum 14 clones including controls per gel.

Infect and harvest 1.5 x 109 insect cells (1 liter culture).

$645

Your cells will be infected by virus at an MOI >1, or as you direct, and harvested 48 hours later. Volume discounts 2-4L 20%, 5-9L 33.3%, 10L or more, 50%.


Purify affinity-tagged protein from 1 liter of culture.

$1495 plus cost of affinity media
$200 per additional liter.

Your protein will be solubilized and purified by one column using an affinity method of your choice.

Electrophoresis and Western blot of subcellular protein fractions

$595 plus cost of first antibody
$250 for additional gels run at the same time

Cells expressing your protein and appropriate controls will be fractionated into cytoplasmic, detergent-soluble and detergent-insolouble fractions, analyzed by gel electrophoresis, blotted and probed with an antibody you provide or using any commercially available antibody. Detection by ECL.

Determine the titer of your virus by limiting dilution.

$95


Purify protein from 1 liter or more of insect culture using your own purification protocol.

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Expression in insect larva

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We also offer expression services in insect larva.